Human Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 (INPP5D) ELISA Kit

A high-quality ELISA kit from Assay Genie designed for the quantitative detection of human Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 (INPP5D) in research samples. The assay is reactive with human serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, and is intended for research use only.

Performance characteristics include a detection range of 0.312-20ng/ml, a sensitivity of 0.15ng/mL, an intra-assay CV of 5.7%, and an inter-assay CV of 9.3%. Recovery averages 91% for serum (range 85-97%) and 93% for plasma (range 87-99%). Linearity has been validated in serum, EDTA plasma, and heparin plasma across multiple dilutions. The kit has been validated for specificity against natural and recombinant human INPP5D.

INPP5D is a phosphoinositide phosphatase that hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), thereby negatively regulating PI3K signaling. Dysregulation of INPP5D has been implicated in cancer, autoimmune disorders, and inflammatory conditions, making this kit relevant to cancer research and investigations of disease pathogenesis.