Human PHLPP ELISA Kit (HUFI07239)

PHLPP (PH domain Leucine-rich repeat Protein Phosphatase) is a serine/threonine phosphatase that controls cell survival by dephosphorylating Akt at Ser473, and the Assay Genie Human PHLPP ELISA Kit enables researchers to quantify this important regulatory protein in human specimens.

The kit supplies a precoated microplate, calibrated standard series, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, TMB substrate, and stop solution for completing a full plate run.

Investigators studying tumor suppressor pathways, insulin signaling, or neurodegeneration use this kit to measure PHLPP concentrations in patient-derived specimens.

Reagents should be stored refrigerated between uses, and conjugate working solutions should be prepared fresh from stock vials immediately before the assay.

The protocol calls for adding 100 uL of diluted sample to each well, incubating for binding, washing, adding conjugate and substrate, and reading absorbance on a standard plate reader.

Results are calculated against the standard curve to determine PHLPP levels across experimental cohorts in cancer biology, diabetes research, and apoptosis pathway studies.