Human PHIP ELISA Kit (HUFI07235)

The Human PHIP (Pleckstrin Homology Domain Interacting Protein) ELISA Kit from Assay Genie enables quantitative measurement of PHIP protein in human research samples. PHIP, also referred to as DCAF14, functions as a nuclear transcriptional regulator and a scaffolding component of the HBO1 histone acetyltransferase complex, where it influences chromatin remodeling, histone H4 acetylation, and downstream gene expression programs tied to cell proliferation.

In metabolic research, PHIP is studied for its role in insulin-receptor signaling and glucose homeostasis through interaction with IRS-1 pleckstrin homology domains. In oncology, PHIP expression has been examined in melanoma, breast, and colorectal carcinoma models, where altered chromatin-regulator levels may reflect tumor subtype or progression. The kit supports these investigations through a 96 Assays plate format suited to mid-throughput biomarker screening in laboratory workflows, with short-term usability at room temperature during assay setup and refrigerated storage between runs.

The assay is intended for research use only and supports in vitro studies in cell biology, signal transduction, and epigenetic regulation. A standard microplate reader capable of absorbance measurements is required, and researchers should consult the lot-specific certificate of analysis and accompanying protocol insert included with each shipment for plate layout, reagent volumes, and recommended dilution schemes for serum-derived samples.