Human PHF6 (PHD finger protein 6) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of PHD finger protein 6 (PHF6) levels in human research samples within the field of epigenetics and nuclear signaling. PHF6 is a protein that plays a critical role in gene regulation and chromatin remodeling, and its dysregulation has been linked to conditions including intellectual disability and certain types of cancer. The assay uses a double-antibody sandwich format with a colorimetric detection method.

The kit is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It has a stated sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. Linearity in serum (n=5) is reported as 85-100% at 1:2, 85-102% at 1:4, and 87-103% at 1:8; EDTA plasma (n=5) at 83-97%, 83-101%, and 89-101%; and heparin plasma (n=5) at 83-97%, 81-100%, and 82-98%. Recovery averages are reported as 98% (serum, n=5), 92% (EDTA plasma, n=5), and 96% (heparin plasma, n=5). Storage is 2-8°C for 12 months.

The procedure follows a standard sandwich ELISA workflow: equilibrate reagents and TMB substrate to room temperature and set up standards, samples, and controls on the pre-coated plate; incubate at 37°C for 90 minutes; add biotin-labeled detection antibody and incubate for 60 minutes; add HRP-Streptavidin (SABC) and incubate for 30 minutes; develop with TMB substrate in the dark for 10-20 minutes; then add stop solution and read absorbance at 450 nm. Synonyms associated with this target include PHD-like zinc finger protein, CENP-31, and KIAA1823.