Human PGD2S/Prostaglandin D2 Synthase, Brain ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of Prostaglandin D2 Synthase (PGD2S) in human samples. The double-antibody sandwich format delivers a sensitivity of 37.5 pg/mL across a standard range of 62.5–4000 pg/mL, and the assay is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Stored at 2–8°C, the kit remains stable for 12 months. Validation data show strong linearity across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with recovery ranges of 85–103% in serum, 91–102% in EDTA plasma, and 86–99% in heparin plasma. The procedure involves a 90-minute primary incubation at 37°C, followed by sequential incubations with biotin-labelled detection antibody and HRP-streptavidin, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm after addition of stop solution.

PGD2S (also known as PTGDS, beta-trace protein, cerebrin-28, or lipocalin-type prostaglandin D synthase; UniProt P41222) is an enzyme central to prostaglandin D2 synthesis and a key mediator of inflammation and immune responses in the brain, placing it within the metabolism research area. With its straightforward protocol and dependable performance, this Assay Genie kit supports studies of neurological inflammation and related disorders.