Human PGAM1/Phosphoglycerate Mutase 1 ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of human Phosphoglycerate Mutase 1 (PGAM1) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format with colorimetric detection, providing a standard range of 0.781–50 ng/mL and a sensitivity of 0.469 ng/mL. PGAM1 is a key glycolytic enzyme implicated in energy metabolism, and its dysregulation has been associated with cancer, metabolic disorders, and neurological conditions, making it a useful biomarker for metabolism research.

Reported performance characteristics include sample recovery of 86–103% (average 91%) in serum, 87–103% (average 94%) in EDTA plasma, and 88–105% (average 98%) in heparin plasma, alongside linearity of 81–104% across 1:2 to 1:8 dilution factors. Reagents are stored at 2–8°C for 12 months, and the workflow uses a biotin-labeled detection antibody with HRP-streptavidin conjugate, TMB color development, and absorbance reading at 450 nm.