Human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3) ELISA Kit (HUFI07256)

PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3) is a bifunctional glycolytic regulator that controls intracellular levels of fructose-2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1. Researchers investigating tumor metabolism, endothelial activation, and cytokine-driven inflammatory signaling rely on quantitative ELISA measurement of PFKFB3 protein to dissect the Warburg effect, angiogenic switching, and stress-response pathways. This quantitative immunoassay from Assay Genie is configured in a microplate format with reagents sufficient for 96 individual determinations, supporting full-plate experimental designs or parallel standard-curve replicates. The kit is intended for research use only, with applications in studies of human cancer cell proliferation, rheumatoid inflammation, and metabolic adaptation, where altered PFKFB3 expression is a documented driver. Investigators studying HIF-1α signaling, AMPK crosstalk, or apoptotic priming will find PFKFB3 quantification valuable for pathway-level interpretation across cell lysates and tissue extracts.