Human PFKFB2 ELISA Kit (HUFI07262)

The Human PFKFB2 ELISA Kit from Assay Genie enables quantitative measurement of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, a bifunctional enzyme that synthesizes and degrades fructose-2,6-bisphosphate, the principal allosteric activator of phosphofructokinase-1 in glycolysis.

PFKFB2 is highly expressed in cardiac and skeletal muscle, where it links glucose utilization to contractile demand, and it is widely studied in tumor metabolism, hypoxia signaling, and insulin-response research. Reconstituted standards and detection antibody should be stored at 4 degrees C, the microplate kept in its sealed pouch with desiccant, and any leftover biological material aliquoted and frozen to limit freeze-thaw cycles across the kit's shelf life. Researchers apply this ELISA to investigate Warburg-effect reprogramming in oncology, cardiac ischemia models, and metabolic-disease biomarker screening using a standard microplate reader workflow.