Human PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1) ELISA KIT (HUFI05490)

Encoded by the PFKFB1 gene, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is a bifunctional metabolic enzyme that controls intracellular levels of fructose-2,6-bisphosphate, a key allosteric regulator of glycolysis. This Assay Genie ELISA kit enables quantitative measurement of human PFKFB1 protein in research samples, supporting studies of glucose metabolism, skeletal muscle physiology, and metabolic disease research.

The kit is configured for 96 Assays per plate and employs an absorbance-based read-out compatible with standard microplate readers. It is intended for research use only and is validated for human samples. Reagents should be stored refrigerated upon arrival to preserve activity across the assay workflow.

This immunoassay tool supports investigations into how PFKFB1 modulates glycolytic flux, particularly in contexts where fructose-2,6-bisphosphate signaling influences cell proliferation, stress response, and tissue-specific energy homeostasis. Researchers studying the PFKFB family of enzymes, including comparisons with PFKFB2, PFKFB3, and PFKFB4 isoforms, can apply this kit to track PFKFB1 expression in cell culture lysates, tissue extracts, and biofluid preparations.