Human Peroxisome proliferator-activated receptor gamma (PPARG) ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the precise measurement of PPARγ levels in human serum, plasma, and cell culture supernatants, delivering accurate and reproducible data for research applications. PPARγ is a key transcription factor involved in the regulation of lipid metabolism, insulin sensitivity, and inflammation, and its dysregulation has been linked to obesity, diabetes, and cardiovascular disorders, making its measurement valuable for studies into the pathogenesis of these conditions.

The assay uses a sandwich ELISA format with colorimetric detection and is reactive with human samples. The detection range is 0.156-10ng/mL and the sensitivity is 0.056ng/mL. Intra-assay CV is 6.1% and inter-assay CV is 11.2%. Linearity has been validated across serial dilutions in serum, EDTA plasma, and heparin plasma, and recovery ranges from 106-118% for serum (average 112%) and 108-120% for plasma (average 114%). The kit is specific for natural and recombinant human peroxisome proliferator-activated receptor gamma and is intended for research use only, with a research area in neurosciences.

Accepted sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Listed synonyms for the target are Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, and NR1C3, and the subcellular localisation is reported in the nucleus and cytoplasm, with redistribution from the nucleus to the cytosol occurring through a MAP2K1/MEK1-dependent manner.