Human Peroxiredoxin-6 (PRDX6) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of Peroxiredoxin-6 (PRDX6) in human serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, with reactivity specific to human samples. Detection is colorimetric and the kit is intended for research use only, including work in the cancer research area where PRDX6 dysregulation has been implicated in disease.

The assay reports a detection range of 15.6-1000pg/mL with a sensitivity of 4.6pg/mL. Precision is documented as an intra-assay CV of 4.7% and an inter-assay CV of 7.5%. Recovery has been validated in serum (average 99%, range 93-105%) and plasma (average 101%, range 95-107%), and linearity has been demonstrated across serial dilutions from 1:2 to 1:16 in serum, EDTA plasma and heparin plasma. Specificity is stated for natural and recombinant human Peroxiredoxin-6.

PRDX6 is described as a bifunctional enzyme involved in redox regulation, capable of reducing H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides, with a role in protection against oxidative injury. It belongs to the AhpC/TSA family (Rehydrin subfamily) and is localised to the cytoplasm, lysosome, cytoplasmic vesicles and lung secretory organelles. The associated UniProt accession is P30041.