Human Peptidase D (PEPD) ELISA Kit

Prolidase, the enzyme encoded by the Peptidase D (PEPD) gene, hydrolyzes dipeptides containing C-terminal proline residues and plays a central role in collagen recycling within connective tissues. This Human Peptidase D (PEPD) ELISA Kit from Assay Genie provides a quantitative immunoassay for measuring PEPD protein levels in serum or plasma specimens, as well as in tissue homogenates prepared from human samples.

The assay is supplied as a 96-assay microplate format with pre-coated wells, a biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop solution, all packaged in individual vials and shipped with detailed protocol documentation. Reagents should be refrigerated between uses to preserve activity, and the kit is designated for research use only in laboratory settings.

Typical research applications include investigations of prolidase deficiency, an inherited disorder of connective tissue, as well as studies of wound healing, fibrosis, and tumor biology where dysregulated PEPD expression has been reported. The assay is compatible with standard microplate reader instrumentation commonly available in immunology and cell biology laboratories, supporting biomarker discovery and enzyme activity studies within broader cell culture research workflows.