Human Pepsin A (PGA3) ELISA Kit

A colorimetric Sandwich ELISA Kit from Assay Genie designed for the accurate and sensitive detection of Human Pepsin A (PGA3), also known as Pepsinogen-3. The assay is intended for human samples including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and is developed for research applications requiring reliable and reproducible results.

The kit states a detection range of 1.56-100ng/mL, a sensitivity of 0.78ng/mL, an intra-assay CV of 4.5% and an inter-assay CV of 8.7%. Specificity is reported for natural and recombinant human Pepsin A-3. The target protein (UniProt P0DJD8) is described as a secreted aspartic-type endopeptidase mapped to chromosomal location 11q12.2, with broad cleavage specificity that prefers bonds involving phenylalanine and leucine.

Pepsin A is a digestive enzyme produced by gastric chief cells that functions in the breakdown of dietary proteins in the stomach. The manufacturer notes that abnormal levels of pepsin A have been associated with gastric disorders such as peptic ulcers, gastroesophageal reflux disease (GERD) and gastritis, supporting research into these conditions and into potential therapeutic interventions. For research use only.