Human PEDF (Pigment Epithelium Derived Factor ) CLIA Kit (HUES01037)

The chemiluminescent (CLIA) detection principle provides several advantages over conventional colorimetric ELISA: a wider dynamic range (typically 3-4 log), a lower limit of detection in the low pg/mL range, and reduced interference from hemolyzed or lipemic samples, which is particularly useful when working with serum from clinical research cohorts.

PEDF (gene symbol SERPINF1) is constitutively expressed in the retinal pigment epithelium, the liver, and adipose tissue, and circulating levels have been investigated as candidate biomarkers in studies of age-related macular degeneration, diabetic retinopathy, prostate and pancreatic cancer, and metabolic syndrome. The 96-well microplate format supports standard curve generation with recombinant PEDF calibrators and is compatible with automated plate luminometers common in core facilities.

Each kit contains a pre-coated microplate, biotinylated detection antibody, lyophilized standards, wash buffer, and chemiluminescent substrate. Reconstituted standards should be aliquoted and stored at -20 °C if not used immediately, while the working substrate is equilibrated to room temperature before use. The protocol typically requires 50 µL of sample per well with a 2-hour primary incubation.

Researchers frequently pair this kit with VEGF-A and endostatin assays to profile pro- versus anti-angiogenic factor balance in conditioned media and ocular fluid specimens. Order online from Assay Genie with worldwide shipping and protocol optimization support.