Human PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit (HUES02641)

The Assay Genie sandwich ELISA kit quantifies human PECAM1/CD31, a 130 kDa transmembrane glycoprotein expressed on endothelial cells, platelets, and leukocytes that mediates leukocyte transendothelial migration and vascular integrity. The colorimetric assay uses a 96-well microplate pre-coated with capture antibody and produces a measurable signal proportional to PECAM1/CD31 concentration in serum, plasma, and other biological fluids. Each kit contains a standard curve, detection antibody, reagents, and substrate solution sufficient for 96 wells. Standard protocol recommends running samples in duplicate, with a working range typically spanning 0.31-20 ng/mL and sensitivity around 0.1 ng/mL. Store all components at 2-8 degrees C, avoid freeze-thaw cycles, and bring reagents to room temperature before use. This ELISA is intended for research use only and supports applications in angiogenesis, cardiovascular disease, inflammation, tumor microenvironment, and vascular biology studies.