Human PDLIM3 ELISA Kit (HUFI07288)

Manufactured by Assay Genie, this PDLIM3 ELISA kit enables quantitative measurement of PDZ and LIM domain 3 (PDLIM3), also known as actinin-associated LIM protein (ALP). PDLIM3 is a cytoskeletal scaffold protein expressed predominantly in cardiac and skeletal muscle, where it interacts with alpha-actinin and other structural components to organize the sarcomere. Researchers apply this kit to characterize PDLIM3 levels in human samples as part of investigations into muscle biology, cardiac development, heart failure, and cytoskeletal remodeling. The kit is supplied in a 96 Assays configuration and includes a pre-coated microplate, biotinylated detection antibody, recombinant PDLIM3 standards, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution needed to generate a standard curve and quantify unknown samples. Protocols call for adding prepared specimens and standards to the plate wells, incubating with detection reagents, and reading the colorimetric signal to calculate PDLIM3 concentration. Store unopened components refrigerated and protect from light until first use. Intended for research use only, the kit supports biomarker discovery, target validation, and basic research into striated muscle gene expression and disease mechanisms.