Human PDK3 ELISA Kit (HUFI07287)

Quantitative PDK3 Immunoassay

Built around the quantitative measurement of Pyruvate Dehydrogenase Kinase 3 (PDK3) in human specimens, this Assay Genie ELISA Kit enables researchers to profile expression of a mitochondrial serine/threonine kinase that phosphorylates the E1α subunit of the pyruvate dehydrogenase complex and gates entry of carbon from glycolysis into the TCA cycle. PDK3 sits at a regulatory node linking nutrient availability, hypoxia signaling, and mitochondrial oxidative metabolism, making it a frequent target in studies of metabolic disease, tumor metabolism, and mitochondrial bioenergetics.

The kit supports 96 Assays and is configured for use with human specimens, employing a matched antibody pair approach for analyte capture and detection. Standard ELISA workflows are supported, including standard curve generation, sample incubation, detection antibody binding, substrate development, and absorbance measurement. Storage of reconstituted standards at refrigerated temperatures is recommended; protect reagents from light and avoid repeated freeze-thaw cycles of working solutions.

Typical research applications include profiling PDK3 expression in cell culture models under hypoxic or pharmacological challenge, comparing PDK3 levels across patient-derived primary samples, and pairing PDK3 quantitation with downstream western blot, flow cytometry, or transcript analyses. The assay is supplied for research use only and is not intended for diagnostic procedures.