Human PDK1 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial) ELISA KIT (HUFI04939)

Assay Genie's PDK1 immunoassay enables quantitative detection of human pyruvate dehydrogenase kinase isozyme 1 in patient-derived biospecimens using a microplate-based absorbance readout. PDK1 is a mitochondrial serine/threonine kinase that phosphorylates the E1α subunit of the pyruvate dehydrogenase complex, inactivating the conversion of pyruvate to acetyl-CoA and directing carbon flux toward glycolysis. Researchers studying tumor metabolism, hypoxia signaling, dichloroacetate pharmacology, and metabolic disorders such as type 2 diabetes rely on this ELISA to track PDK1 expression across treatment conditions. The kit ships with a pre-coated antibody plate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated protein standards, and TMB substrate in a sufficient quantity for 96 individual determinations. A sample volume of 100 uL is loaded per well, with the standard curve prepared in the supplied assay diluent. Store the kit refrigerated at 4°C upon arrival, keep the standard aliquoted to limit freeze-thaw cycles, and protect the TMB substrate from light exposure to preserve signal integrity.