Human PDGFD (Platelet Derived Growth Factor D) ELISA Kit (HUES03105)

Each Assay Genie kit ships with a pre-coated 96-well microplate, lyophilized recombinant human PDGFD standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and substrate solution. The protocol requires 100 µl of sample or standard per well, with sequential incubations totaling approximately 3.5 hours at room temperature before the final substrate reaction. The standard curve spans a broad dynamic range, allowing quantification of both low-abundance and higher-titer samples from cell culture supernatant, serum, or plasma.

The kit is validated for research use only and is routinely cited in studies of tumor angiogenesis, pulmonary fibrosis, and vascular remodeling, where PDGFD-driven PDGF receptor-beta signaling plays a documented role. Compatible with any standard microplate reader equipped with a 450 nm filter, the assay delivers reproducible results when samples are aliquoted and stored frozen at -80°C prior to analysis, and reconstituted reagents are kept at 2-8°C between uses. Bring all reagents to room temperature before initiating the assay to ensure consistent kinetics across the plate, and avoid repeated freeze-thaw cycles of the reconstituted standard to preserve binding activity.