Human PDE4D (Phosphodiesterase 4D, cAMP Specific) CLIA Kit (HUES01271)

Assay Genie offers a chemiluminescence-based immunoassay designed for the quantitative determination of Human PDE4D (Phosphodiesterase 4D, cAMP Specific). The kit operates on a Sandwich-CLIA principle, with a pre-coated microplate that captures the target analyte. Standards or samples are combined with a specific antibody, followed by a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. Signal is generated as Relative Light Unit (RLU) values that correlate positively with Human PDE4D concentration and are read on a chemiluminescence immunoassay analyser.

The assay delivers a sensitivity of 37.50 pg/mL and a detection range of 62.50-4000 pg/mL, with a total assay time of 4.5h. It is intended for use with human samples, including serum, plasma, tissue homogenates, cell lysates, and other biological fluids. The kit specifically recognizes Human PDE4D in samples, with no significant cross-reactivity or interference between Human PDE4D and analogues observed. Reproducibility is reported as both intra-CV and inter-CV < 15%.

This kit supports research in Cell Biology and is suited to studies of cAMP-regulated signalling. According to Assay Genie, dysregulation of PDE4D has been implicated in inflammatory disorders, immune system dysfunction, and neurodegenerative conditions, making accurate measurement of PDE4D relevant to investigations of disease pathogenesis and the development of targeted therapies.