Human PDCD6IP (Programmed cell death 6-interacting protein) ELISA Kit (HUFI08652)

The Human PDCD6IP ELISA Kit supplied by Assay Genie provides quantitative measurement of Alix, a 95 kDa cytoplasmic scaffold protein encoded by the PDCD6IP gene on chromosome 17. Alix participates in programmed cell death signaling, endosomal sorting complexes required for transport (ESCRT) machinery assembly, multivesicular body formation, and viral budding at the plasma membrane.

Oncology investigators use this assay to evaluate Alix levels in tumor lysates and circulation as a marker of apoptotic dysregulation and tumor progression. Neuroscience laboratories quantify PDCD6IP concentrations in patient samples to correlate with neurodegenerative disease severity, while virology studies examine Alix involvement in HIV-1 release pathways and other enveloped virus budding mechanisms.

The microplate-based protocol uses a sample volume of 100 uL per well, a chromogenic HRP substrate readout, and standard laboratory incubation steps. Reconstituted components remain stable when aliquoted and stored at refrigerated temperatures, with long-term preservation below -20 degrees C and protection from light for the working substrate solution. Each kit ships as a single-use pack with pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, dilution buffer, wash buffer, TMB substrate, and stop solution.