Human PCYT2 ELISA Kit (HUFI07311)

About PCYT2 (Phosphate Cytidylyltransferase 2, Ethanolamine): The PCYT2 gene encodes ethanolamine-phosphate cytidylyltransferase, the rate-limiting enzyme of the Kennedy pathway responsible for de novo phosphatidylethanolamine (PE) biosynthesis in mammalian cells. PE is the second most abundant glycerophospholipid in eukaryotic membranes and contributes to autophagy, mitochondrial function, and membrane curvature.

Assay Genie supplies this colorimetric microplate kit for quantitative measurement of PCYT2 protein in human serum and plasma specimens. The capture antibody is human-specific, with no significant cross-reactivity observed with rat or mouse PCYT2 orthologs, supporting clean signal discrimination in translational studies. Each microplate contains 96 pre-coated wells, paired with biotinylated detection antibody, streptavidin-HRP conjugate, and calibrated reference standards for reproducible quantification across runs.

Storage and handling: shipped refrigerated on gel packs; reconstituted components should be aliquoted and stored frozen to limit freeze-thaw cycles, and the working detection solution should be protected from light. Researchers investigating alcoholic liver disease, non-alcoholic fatty liver disease, neurodegeneration, or phospholipid remodeling pathways can apply this assay to clinical serum and plasma cohorts for biomarker discovery and mechanistic lipid biology studies.