Human PCNA PharmaGenie ELISA Kit (SBRS0975)

The PCNA ELISA kit from Assay Genie quantifies Proliferating Cell Nuclear Antigen, a 36 kDa nuclear protein that acts as the processivity factor for DNA polymerase delta during DNA replication. PCNA expression peaks during S-phase of the cell cycle, making it a widely studied biomarker for cell proliferation in oncology, toxicology, and tissue regeneration research.

The colorimetric readout is configured to quantify PCNA from cell culture supernatant, plasma-derived, and serum-derived specimens, with reactivity validated against human, mouse, and rat PCNA. Researchers commonly apply this type of immunoassay when monitoring proliferative responses in tumor biopsies, evaluating compound effects on cell division kinetics, or characterizing regenerative processes in tissue-derived samples.

The microplate workflow supports parallel processing of multiple samples, with absorbance readings captured on a standard plate reader. Unopened reagents should be refrigerated between uses, with longer-term storage of individual components handled as indicated in the product insert. Each kit contains pre-coated wells, detection antibody, standards, and assay buffer sufficient for the stated number of determinations, intended for research use only and not validated for diagnostic or clinical applications.