Human PCI (Procollagen I) ELISA Kit (HUES01448)

Designed by Assay Genie for the precise measurement of procollagen I levels in human samples, this Sandwich ELISA Kit uses a colorimetric detection method. The assay is intended for use with serum, plasma, and other biological fluids, and operates on a Sandwich-ELISA principle with a total assay time of 3 h 30 min. Procollagen I is a key precursor involved in the synthesis and formation of collagen, the main structural protein in the human body.

Performance specifications include a sensitivity of 37.5 pg/mL, a detection range of 62.5-4000 pg/mL, and a recovery of 80%-120%. The required sample volume is 100 μL. The workflow uses a biotinylated detection antibody specific to the target protein, followed by Avidin-Horseradish Peroxidase (HRP) conjugate, with the substrate reaction terminated by stop solution and the optical density measured at 450 nm ± 2 nm. The kit recognizes Human PCI in samples with no significant cross-reactivity or interference between Human PCI and analogues, and is intended for storage at 2-8°C. Research areas include Signal Transduction and Stem Cells.