Human PCDHGB3 ELISA Kit (HUFI07320)

Protocadherin Gamma Subfamily B, 3, a calcium-dependent transmembrane glycoprotein encoded within the protocadherin gamma cluster, can be quantified in human specimens using the Assay Genie Human PCDHGB3 ELISA Kit. This microplate-based research use only assay supports neuroscience and molecular oncology investigations into neuronal differentiation, synaptic partner matching, axon guidance, and altered protocadherin expression associated with tumorigenesis.

PCDHGB3 is primarily expressed in neuronal tissues, where it contributes to cell-cell recognition and the formation of specific synaptic connections during central nervous system maturation. Investigators can apply the kit to profile PCDHGB3 levels, explore its emerging role as a candidate biomarker in neurological disorders, and characterize membrane protein expression across patient-derived samples.

The kit ships with pre-coated microplate strips, calibrated recombinant protein standards, matched antibody pairs, detection reagents, wash concentrates, and a detailed protocol insert. The dual-antibody capture workflow immobilizes target protein from test specimens and uses a conjugated detection antibody to generate a measurable signal proportional to PCDHGB3 concentration. Standard curve generation enables accurate interpolation of unknown sample values.

Reagent components should be stored refrigerated upon arrival and protected from light, with reconstituted standards aliquoted to minimize freeze-thaw cycles across the workflow.