Human PCDHB16/Protocadherin Beta 16 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of Human PCDHB16 (Protocadherin Beta-16) in human biological samples including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The kit uses a double antibody sandwich format with colorimetric detection, and absorbance is read at 450 nm.

PCDHB16 is a member of the protocadherin beta family and is involved in cell adhesion and communication in the central nervous system. Dysregulation of PCDHB16 has been associated with neurological disorders including autism and schizophrenia, making this kit a useful tool for research into these conditions. Reported recovery values average 95% for serum, 95% for EDTA plasma, and 96% for heparin plasma, with linearity ranges generally between 83% and 105% across tested dilutions of these matrices.

Reagents should be stored at 2-8°C for 12 months. The procedure involves a 90-minute primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-Streptavidin incubation, TMB substrate development in the dark for 10-20 minutes, and a stop reaction read at 450 nm. Sample preparation protocols are provided for serum, plasma, tissue homogenates, cell culture supernatants, and cell lysates.