Human PCDH15/Protocadherin 15 ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative measurement of human PCDH15 (Protocadherin 15) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody sandwich format with colorimetric detection supports research in cell biology, including studies of cell–cell adhesion and communication in tissues such as the inner ear and retina, and investigations into conditions such as Usher syndrome and nonsyndromic hearing loss where PCDH15 mutations are implicated.

According to the manufacturer's specifications, the assay has a sensitivity of 0.188 ng/mL and a measurable range of 0.313–20 ng/mL. Reported linearity for serum (n=5) is 96–105%, 86–103% and 87–103% at 1:2, 1:4 and 1:8 dilutions respectively; EDTA plasma (n=5) shows 95–101%, 82–92% and 92–99%; and heparin plasma (n=5) shows 87–96%, 80–87% and 83–100%. Recovery ranges are 91–103% (average 100%) for serum, 90–103% (average 96%) for EDTA plasma, and 89–103% (average 98%) for heparin plasma. The kit is stored at 2-8°C for 12 months.

The workflow involves equilibrating reagents and TMB substrate to room temperature, loading standards, samples and blanks for a 90-minute primary incubation at 37°C, adding biotinylated detection antibody for 60 minutes at 37°C, incubating with HRP-Streptavidin (SABC) for 30 minutes at 37°C, developing colour with TMB substrate in the dark for 10–20 minutes, and reading absorbance at 450 nm after adding stop solution.