Human PBX1 ELISA Kit (HUFI07325)

PBX1 (Pre-B-cell leukemia homeobox 1) is a homeodomain transcription factor essential during embryonic hematopoietic development and frequently implicated in leukemia research because of the t(1;19) chromosomal translocation that produces the E2A-PBX1 fusion oncoprotein. This quantitative immunoassay from Assay Genie is configured to measure PBX1 protein in human serum or plasma-derived research samples using a two-antibody ELISA procedure read on a standard absorbance plate reader.

Each kit contains sufficient reagents for 96 Assays, including a pre-coated plate, biotinylated detection antibody, streptavidin-HRP conjugate, calibration standards, assay diluent, wash buffer, and substrate solution. Researchers investigating aberrant transcription factor expression in B-cell acute lymphoblastic leukemia, myeloid differentiation programs, or normal hematopoietic stem cell signaling will use the kit to generate concentration data that can be correlated with transcript-level findings.

The recommended storage condition is refrigeration at 4°C between uses, with all components equilibrated to room temperature before each run. The procedure follows a standard ELISA workflow with absorbance read after stop solution addition, and a standard curve generated from the kit's calibrators is used to interpolate unknown sample concentrations.