Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit

The PNPLA3 protein, also called adiponutrin, sits at the center of hepatic lipid metabolism research because it acts as a triglyceride hydrolase in hepatocytes and harbors the well-studied I148M variant linked to fatty liver disease progression. This Assay Genie kit delivers a quantitative colorimetric immunoassay built around the 96-assay plate format, giving laboratories working on non-alcoholic fatty liver disease, alcoholic liver disease, or metabolic liver injury a route to measure endogenous human PNPLA3 directly in tissue homogenates and prepared cell lysates. Researchers studying adipogenesis, hepatic steatosis, or the genetic drivers of lipid droplet accumulation in liver biopsies will find the plate format practical for moderately sized study cohorts, with each well read on a standard absorbance plate reader. The reagents are configured for in vitro research use only and arrive as a complete ready-to-run kit, removing the need to source matched antibody pairs and standards separately. Because PNPLA3 expression rises in steatotic and inflamed liver tissue, the assay supports comparative work between healthy donor samples and patient-derived specimens, including longitudinal sampling in studies of disease progression or treatment response. For labs that need to correlate genotype at the I148M locus with actual protein abundance in liver tissue, this kit pairs naturally with DNA extraction from the same sample set, since the functional polymorphism exerts its strongest effects at the protein level in hepatocytes.