Human Patatin-like phospholipase domain-containing protein 2 (PNPLA2) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of human Patatin-like phospholipase domain-containing protein 2 (PNPLA2) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay uses a colorimetric detection method and is intended for research use only, supporting studies within the cardiovascular research area.

Key performance specifications quoted from the manufacturer: a detection range of 0.625-40ng/mL, sensitivity of 0.265ng/mL, intra-assay CV of 5.6%, and inter-assay CV of 9.1%. Reported recovery is 91% (range 85-97%) for serum and 93% (range 87-99%) for plasma. Linearity data are provided for serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16.

PNPLA2, also known by synonyms including Adipose triglyceride lipase, Desnutrin, ATGL, IPLA2-zeta and TTS2.2, catalyses the initial step in triglyceride hydrolysis in lipid droplets. The kit provides a tool for investigating the enzyme's role in energy homeostasis and metabolic disorders such as obesity and non-alcoholic fatty liver disease.