Human PARP1 ELISA Kit

From Assay Genie, this Sandwich ELISA is designed for the quantitative measurement of Poly (ADP-ribose) polymerase 1 (PARP1) levels in human samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The kit uses a colorimetric detection method with a double-antibody sandwich format, delivering high sensitivity and specificity for reproducible results.

Key performance specifications include a sensitivity of 0.375 ng/mL and an assay range of 0.625–40 ng/mL. PARP1 is a key enzyme involved in DNA repair and maintenance, as well as in cellular processes including cell division and gene expression regulation. Its dysregulation has been linked to cancer, inflammatory disorders, and neurodegenerative conditions, highlighting its relevance as a potential biomarker for disease diagnosis and therapy development. The kit is suited to research in epigenetics and nuclear signaling.

Validated linearity across serum (86–96% at 1:2, 85–105% at 1:4, 87–103% at 1:8), EDTA plasma (82–101%, 93–98%, 82–96%) and heparin plasma (93–100%, 80–99%, 91–99%) dilutions. Recovery ranges are 86–103% for serum (average 94%), 90–97% for EDTA plasma (average 92%) and 86–102% for heparin plasma (average 93%). The kit should be stored at 2–8°C for 12 months.