Human Park7/DJ-1 PharmaGenie ELISA Kit (SBRS0971)

Park7/DJ-1 is a multifunctional redox-sensitive protein encoded by the PARK7 gene, originally identified as an oncogene and later recognized as a causal gene for autosomal recessive early-onset Parkinson's disease. The Assay Genie immunoassay supports neurodegeneration research by quantifying Park7/DJ-1 concentrations in cell culture supernatants, plasma or serum specimens, and tissue lysates, enabling biomarker studies in Parkinson's disease, oxidative stress modeling, and mitochondrial dysfunction investigations.

Under oxidative stress, DJ-1 shifts to an acidic isoform (pI approximately 5.7) and forms homodimers that protect neurons against hydrogen peroxide-induced cell death. Researchers use this kit with a 100 ul sample volume to monitor DJ-1 levels in dopaminergic cell models, patient samples, and post-mortem brain tissue, correlating expression changes with disease progression or therapeutic intervention. The antibody pair is specific to the human Park7/DJ-1 target, supporting studies in clinical cohorts and cell-based Parkinson's disease models.

Each kit ships with a pre-coated microplate, lyophilized recombinant DJ-1 standard, biotin-conjugated detection antibody, streptavidin-HRP working solution, wash buffer concentrate, and TMB substrate. Store the sealed kit refrigerated at 4°C and protect from light; reconstituted standards and working solutions should be aliquoted and stored frozen at -20°C to limit freeze-thaw cycles. Intended for research use only, the assay enables in vitro measurement of native and recombinant human Park7/DJ-1 protein in experimental samples.