Human PARD3 ELISA Kit (HUFI07346)

The Assay Genie PARD3 immunoassay enables quantitative measurement of Par-3 Family Cell Polarity Regulator in human samples using a two-antibody capture enzyme-linked immunosorbent approach. PARD3 is a scaffold protein that organizes apical-basal polarity in epithelial cells, recruits the PAR6/aPKC complex to tight junctions, and regulates asymmetric cell division. Dysregulation of PARD3 expression and copy number has been documented in lung adenocarcinoma, breast carcinoma, glioblastoma, and esophageal squamous cell carcinoma, where it correlates with tumor grade, invasion potential, and patient prognosis. Researchers use the kit for in vitro studies of polarity signaling, junction assembly, and oncogenic transformation.

The kit is supplied in a pack configured for 96 determinations, sufficient for standard curve replicates and multiple sample measurements on a single microplate. All reagents arrive ready-to-use in individually labeled vials to preserve reagent integrity through repeated thaw cycles. Researchers should aliquot the conjugate and standards upon first use and store the kit refrigerated to maintain binding activity across the full shelf life. Standard curve concentrations span the dynamic range expected for circulating biomarker studies, and the absorbance-based read-out integrates with common plate readers in the laboratory.

Researchers investigating epithelial-mesenchymal transition, neuronal polarity, or asymmetric division in stem cell populations will find this kit useful for profiling endogenous PARD3 levels in cell lysates and conditioned media. The kit is intended for research use only and is not validated for diagnostic procedures.