Human PARC PharmaGenie ELISA Kit (SBRS0970)

PARC (p53-Associated Parkin-like Cytoplasmic protein, also designated CUL9) is quantified by this immunoassay from Assay Genie across human plasma samples, serum specimens, and cell culture supernatant fractions. PARC functions as a cytoplasmic ubiquitin ligase that interacts directly with the tumor suppressor p53, modulating p53 stability and downstream transcriptional activity, which positions this assay as a useful tool for tumor suppressor pathway research, DNA damage response studies, and broader oncology investigations.

The kit is supplied in a 96-assay microplate format with a colorimetric readout obtained on a standard plate reader. Reagents ship refrigerated; unopened vials and bottle components should remain stored cold and protected from light until first use, with working antibody and standard dilutions prepared fresh before each run. The product is intended for research use only and is not validated for diagnostic procedures.

Each pack contains pre-coated plate strips, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated recombinant PARC standards, wash buffer concentrate, substrate solution, and stop reagent. The included protocol involves a sequential incubation workflow with intervening wash steps, and the recombinant standards support construction of a calibration curve for interpolating endogenous PARC concentrations in conditioned media and clinical research specimens.