Human PAR3/Protease Activated Receptor 3 ELISA Kit

A high-quality sandwich ELISA from Assay Genie designed for the quantitative detection of human PAR3 (Protease-Activated Receptor 3) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit employs a double-antibody sandwich format with colorimetric readout, offering a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL.

PAR3 is a key cell-signalling protein activated by proteases and implicated in inflammation, blood clotting, and cell migration. Dysregulation of PAR3 has been linked to cancer, cardiovascular disorders, and neurological conditions, supporting its use in cardiovascular and broader disease-pathology research. Reported assay linearity spans 81–105% across serum, EDTA plasma, and heparin plasma dilution series, with average recoveries of 96% (serum), 92% (EDTA plasma), and 98% (heparin plasma).

Reagents are stored at 2-8°C for 12 months. The supplied microplate is pre-coated, and the workflow uses a biotin-labelled detection antibody with HRP-Streptavidin (SABC) and TMB substrate, with absorbance read at 450 nm after stop-solution addition. Sample-specific preparation protocols are provided for serum, plasma, tissue homogenate, cell culture supernatant, and cell lysate.