Human PAR2 (Protease Activated Receptor 2) ELISA Kit (HUES02273)

Beyond the standard sandwich ELISA workflow, this kit (from Assay Genie) supports quantitative measurement of PAR2 (also known as F2RL1 or GPR11), a 397-residue, seven-transmembrane class A G-protein coupled receptor irreversibly activated by proteolytic cleavage of its N-terminus at the R36/S37 site. Endogenous activators include trypsin, tryptase, matriptase, cathepsin S, granzyme A, factor VIIa, and neutrophil elastase; the synthetic peptide SLIGKV can also elicit receptor activation independent of cleavage. Downstream signaling proceeds largely through Gq and G12/13 coupling, with secondary Gi involvement, mobilizing intracellular calcium and engaging MAPK/ERK cascades. The biotin-streptavidin-HRP detection chain provides signal amplification, with typical sample volumes of 50-100 µL per well applied to the pre-coated 96-well microplate. Cell culture supernatants are usually assessed directly or after dilution in assay diluent, while serum and plasma samples may require dilution to fall within the standard curve range generated from the recombinant PAR2 calibrator. For reproducible data, equilibrate all reagents to room temperature before use, run samples in duplicate or triplicate, and re-assay any sample whose optical density falls above the highest standard at a higher dilution. Absorbance is read at 450 nm (with an optional 570 nm reference correction) on a standard plate reader, and the assay typically completes in 3-4 hours. PAR2 is widely expressed on endothelial, epithelial, immune, and smooth muscle cells, supporting its investigation in tumor microenvironments, rheumatoid synovium, inflammatory bowel disease biopsy material, and airway inflammation models. The kit is intended for research use only and is not validated for clinical diagnostic applications.