Human PAQR7/Progestin and AdipoQ Receptor Family Member VII ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of PAQR7 (Progestin and AdipoQ Receptor Family Member VII) levels in human specimens. The assay uses a double antibody sandwich format with colorimetric detection, and is suitable for use with serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. PAQR7 plays a role in hormone signaling, lipid metabolism, and cell proliferation, and its dysregulation has been implicated in diseases such as cancer, obesity, and metabolic disorders, making it a valuable biomarker for research studies.

Reported linearity for serum spans 87–104% across dilutions (n = 5), with EDTA plasma at 91–102% and heparin plasma at 94–100% at the 1:2 dilution. Recovery is reported at an average of 95% for serum, 96% for EDTA plasma, and 97% for heparin plasma. Reagents should be stored at 2–8°C for up to 12 months.

The protocol involves equilibrating reagents and TMB substrate to room temperature, incubating the plate at 37°C for 90 minutes for primary antigen binding, followed by biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution addition. The kit includes a pre-coated dismountable microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, plate sealers, and a technical manual.