Human Pappalysin-1 (PAPPA) ELISA Kit

Assay Genie offers a sandwich ELISA designed for the quantitative measurement of human Pappalysin-1 (PAPPA), also known as Pregnancy-associated plasma protein A (PAPP-A). The assay is intended for research use only and is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

According to the manufacturer's specifications, the kit has a detection range of 62.5-4000 pg/mL and a sensitivity of 31.2 pg/mL. Reported precision values are an intra-assay CV of 6.1% and an inter-assay CV of 10.4%. The assay is specific for natural and recombinant human Pappalysin-1, and PAPPA is a secreted metalloproteinase that cleaves IGFBP-4 and IGFBP-5, releasing bound IGF; IGFBP-4 cleavage is dramatically enhanced by IGF, while IGFBP-5 cleavage is slightly inhibited by it.

Listed synonyms include Insulin-like growth factor-dependent IGF-binding protein 4 protease, IGF-dependent IGFBP-4 protease, PAPA, DIPLA1, PAPPA1, ASBABP2 and IGFBP-4ase, and the associated UniProt entry is Q13219. The target is relevant to research in cell biology, including studies of female pregnancy, cellular differentiation and proteolysis.