Human Pappalysin-1/PAPP-A ELISA Kit

Designed for the precise quantification of Pregnancy-Associated Plasma Protein A (PAPP-A) in human biological samples, this colorimetric Sandwich ELISA kit from Assay Genie delivers high sensitivity and specificity for research applications. PAPP-A is described as a vital enzyme involved in the regulation of insulin-like growth factor binding proteins, impacting cellular processes such as growth, development, and tissue repair. Its dysregulation has been linked to cardiovascular diseases, cancer, and pregnancy complications, highlighting its potential value as a biomarker for disease diagnosis and treatment monitoring.

The assay is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It offers a sensitivity of 0.375 mIU/mL with a working range of 0.625–40 mIU/mL (1 ng = 2 mIU), and the kit is intended for storage at 2–8°C for up to 12 months. Assay performance has been validated across serum, EDTA plasma, and heparin plasma, with reported linearity and recovery data demonstrating reliable results in each matrix. The workflow follows a sandwich ELISA format using a biotin-labeled detection antibody, HRP-streptavidin conjugate, and TMB substrate, with absorbance read at 450 nm.