Human PAPD5 ELISA Kit (HUFI07338)

This in vitro research immunoassay enables quantitative measurement of human PAPD5 protein from biological specimens. PAPD5 (Poly(A) Polymerase Domain Containing 5, also called TENT4B) is a non-canonical terminal nucleotidyltransferase that catalyzes guanylation of mRNA poly(A) tails and contributes to mRNA stability, the TENT-GAN degradation pathway, and DNA double-strand break repair through interaction with the shieldin complex.

Distributed by Assay Genie, the kit supports investigations into post-transcriptional gene regulation, tumor biology, and the cellular response to genotoxic stress, where dysregulated PAPD5 activity has been linked to immune checkpoint biology and chemotherapy resistance. The colorimetric assay workflow is compatible with standard laboratory plate readers, and all components are supplied for research use only in laboratory settings.

Each kit is supplied as a complete pack including pre-coated microplate strips, biotinylated detection antibody, recombinant PAPD5 standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. Working dilutions should be prepared fresh; reconstituted standards and detection antibody should be aliquoted and stored frozen, with repeated freeze-thaw cycles avoided, while kit components remain protected from light until use.