Human PANK2 ELISA Kit (HUFI07350)

The Pantothenate Kinase 2 (PANK2) enzyme is a mitochondrial kinase that catalyzes the first committed step in coenzyme A biosynthesis from pantothenate (vitamin B5). Loss-of-function mutations in PANK2 cause Pantothenate Kinase-Associated Neurodegeneration (PKAN), the most common form of Neurodegeneration with Brain Iron Accumulation (NBIA), making quantitative measurement of PANK2 protein valuable for research into mitochondrial metabolism, iron handling, and movement disorders.

This ELISA kit from Assay Genie is supplied as a 96 Assays format and is intended for research use only, applied in vitro. It enables investigators studying neurodegeneration, CoA pathway biochemistry, and PKAN disease models to quantify human PANK2 in experimental samples. The immunoassay provides a quantitative read-out suitable for studies of patient-derived cell lines, postmortem brain tissue homogenates, and animal model samples engineered to express the human target.

Beyond its obvious relevance to PKAN, PANK2 quantification supports broader investigations into cellular energy metabolism, acyl carrier protein function, and the crosstalk between CoA pools and oxidative stress. Researchers examining pantothenate supplementation strategies, mitochondrial dysfunction in Parkinson's disease, or the role of iron-sulfur cluster disruption in basal ganglia neurodegeneration may also find this kit useful. Each kit is supplied with the reagents required to run the assay in a microplate, and components should be handled according to the supplied insert to preserve activity across the full plate.