Human Pancreatic prohormone/PPY ELISA Kit

Designed by Assay Genie for research use, this ELISA kit is configured for the quantitative measurement of human pancreatic prohormone (PPY) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay format is competitive ELISA with the plate coated with antigen, and it reports a sensitivity of 28.125 pg/mL with a working range of 46.875–3000 pg/mL.

Annotated synonyms for the target protein include PPY, INN, Obinepitide, PNP, PP, pancreatic polypeptide, pancreatic polypeptide Y, and pancreatic prohormone. The associated UniProt accession is P01298. Kit components are intended for storage at 2–8°C in a sealed state; cryopreservation is not advised.

The kit is supplied with a dismountable ELISA microplate, lyophilized standard, 100X biotin-labeled antibody concentrate, 100X HRP-streptavidin conjugate (SABC), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, 25X wash buffer, plate sealer, and a technical manual. After substrate incubation, stop solution is added and absorbance is measured at 450 nm using a microplate reader.