Human PAH/Phenylalanine Hydroxylase ELISA Kit

Designed for the accurate measurement of phenylalanine hydroxylase (PAH) levels in human samples, this Sandwich ELISA from Assay Genie delivers high sensitivity and specificity for reliable, reproducible results. Phenylalanine hydroxylase is a key enzyme involved in the metabolism of the amino acid phenylalanine, and mutations in the PAH gene can lead to phenylketonuria (PKU), a genetic disorder that results in the accumulation of phenylalanine in the body. The kit supports research into PKU mechanisms and potential treatment strategies by enabling quantification of phenylalanine hydroxylase in human samples.

Validated sample matrices include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a sensitivity of 46.875 pg/mL with a measuring range of 78.125-5000 pg/mL and uses a colorimetric detection method. The kit is stored at 2-8°C for 12 months. Linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma. The workflow uses a biotin-labeled detection antibody followed by HRP-streptavidin binding, TMB substrate for colour development, and absorbance measurement at 450 nm.