Human PAFAH1B1 (Platelet-activating factor acetylhydrolase IB subunit alpha) ELISA KIT (HUFI04956)

The Assay Genie human PAFAH1B1 ELISA kit targets LIS1, the regulatory alpha subunit (approximately 45 kDa) of platelet-activating factor acetylhydrolase type IB. Researchers use this quantitative immunoassay to measure PAFAH1B1 protein levels in human research specimens, supporting investigations of neuronal migration, classical lissencephaly, and Miller-Dieker syndrome, where LIS1 haploinsufficiency is known to disrupt dynein-driven nucleokinesis and radial glial scaffolding during cortical development.

The kit ships with a precoated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, concentrated wash buffer, and recombinant PAFAH1B1 standards. The workflow follows standard immunoassay practice: dispense sample and standards into coated wells, incubate, wash, add the detection antibody, wash again, add conjugate, develop color, stop the reaction, and read absorbance on a plate reader. Researchers commonly pair this assay with western blot confirmation or qPCR profiling of PAFAH1B1 transcripts in neuronal cell culture models, including differentiating iPSC-derived cortical neurons, to cross-validate protein-level findings.

For storage, unopened kit components should be refrigerated until first use. Reconstituted standards and any unused aliquoted samples should be frozen promptly and kept protected from light to limit repeated freeze-thaw cycles. The reagents are supplied for research use only and are not intended for diagnostic procedures.