Human PACT ELISA Kit (HUFI07371)

Quantitative measurement of Protein Activator of PKR (PACT, also known as PRKRA) in human biological specimens is supported by the Assay Genie immunoassay, supplied as a 96-test ELISA configuration with colorimetric readout. PACT is a double-stranded RNA-binding protein that activates the interferon-induced protein kinase PKR during cellular stress responses and has been studied in the context of viral defense, apoptosis regulation, and certain neurodegenerative movement disorders.

The kit provides pre-coated microplate wells along with the reagents needed to generate a standard curve and quantify PACT in cell lysates and tissue homogenate preparations. The assay follows a two-antibody capture format in which the target protein is sandwiched between an immobilized capture antibody and a detection antibody conjugated to an enzyme that produces a measurable color change proportional to PACT concentration.

Intended for research use only, the kit assists investigators examining interferon signaling pathways, stress granule biology, and the molecular mechanisms underlying PKR-related disorders. Each kit is shipped with a complete protocol, standard diluent, detection reagents, wash buffer, and substrate solution, allowing laboratories to process a full plate of unknown samples alongside the standard curve in a single experimental session.