Human p35 ELISA Kit (HUFI08668)

The Assay Genie immunoassay kit is built around human p35, the 35 kDa cytokine subunit encoded by the IL12A gene that pairs with p40 to form interleukin-12 and with EBI3 to assemble interleukin-35. Laboratories working on Th1-mediated inflammation, infectious disease immunology, and tumor microenvironment signaling apply this microplate-format kit to quantify soluble p35 in human research specimens, with reagents sufficient for 96 individual determinations. The workflow uses a matched antibody pair: pre-coated capture wells bind p35 from added samples, a biotinylated detection antibody binds the immobilized target, a streptavidin-enzyme conjugate is added, and chromogenic substrate produces a measurable absorbance signal on a standard plate reader. Intended for research use only, the kit is applied across oncology, autoimmune disease, and transplantation research where cytokine profiling and immune-cell cross-talk are of interest. Reconstituted standards and prepared samples are handled per standard immunoassay protocol, and kit components should be kept refrigerated to preserve capture antibody and conjugate activity through the labeled expiration date.