Human P2 (Myelin Protein 2) ELISA Kit (HUES02840)

Supplied by Assay Genie, this quantitative sandwich ELISA is built around a pre-coated 96-well microplate and uses a biotinylated capture antibody paired with streptavidin-HRP for signal generation. After incubation with standards or diluted samples, wells are washed, developed with TMB substrate, and the reaction is stopped before absorbance is read at 450 nm with a 630 nm reference filter. The protocol typically completes in roughly 3 to 4 hours and accommodates sample volumes of 100 µL per well, with a standard curve spanning the low pg/mL range to allow sensitive detection of PMP2 in serum, plasma, cerebrospinal fluid and cell culture supernatants. Reagents should be equilibrated to room temperature before use, and any unused wells stored sealed with desiccant at 2–8 °C remain stable for the shelf life of the kit. Each lot is validated for intra- and inter-assay precision, and the assay is restricted to research use only, not for diagnostic procedures. Bring all kit components to room temperature, protect from light, and avoid repeated freeze-thaw cycles of any frozen reagents to preserve antibody activity; samples may be aliquoted and frozen for long-term storage prior to assay.