Human Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

This colorimetric OxLDL ELISA kit from Assay Genie enables quantitative measurement of oxidized low density lipoprotein in human-derived specimens. Oxidized LDL is a well-established biomarker in atherosclerosis and cardiovascular disease research, and is implicated in foam cell formation, endothelial dysfunction, and inflammatory plaque progression. Researchers studying lipid metabolism, oxidative stress, and vascular pathology use OxLDL measurements to evaluate disease models and therapeutic interventions.

The kit is supplied as a complete immunoassay package with a pre-coated plate, calibration standards, detection antibody, wash buffer, substrate, stop solution, and protocol. Applicable sample matrices include human serum samples, plasma preparations, tissue homogenates, cell lysates, and cell culture supernatant preparations. Researchers should follow the included protocol for standard curve preparation, sample dilution optimization, and incubation times to ensure accurate quantification.

Workflow: load standards and samples onto the plate, incubate, perform washes, add detection reagent, develop signal with substrate, stop the reaction, and read absorbance with a microplate reader. Concentrations are interpolated from the standard curve. Reagent storage conditions should follow the manufacturer's specifications; allow refrigerated components to reach ambient temperature before use. For research use only, not intended for diagnostic procedures.

Each package contains materials sufficient for the stated number of determinations as described in the product specifications. Technical documentation and troubleshooting guidance are available from Assay Genie. The kit supports immunology, vascular biology, and metabolic disease research applications across academic and pharmaceutical laboratory settings.