Human OXCT1 ELISA Kit (HUFI07385)

Built for quantitative detection of 3-oxoacid CoA-transferase 1 (OXCT1), the mitochondrial succinyl-CoA:3-oxoacid CoA-transferase (SCOT) that catalyzes the rate-limiting step of extrahepatic ketone body utilization, the Assay Genie Human OXCT1 ELISA Kit supports research on hepatic ketogenesis, fasting physiology, diabetic ketoacidosis, and inherited SCOT deficiency. The 96 Assays format kit supplies the antibody-coated microplate, reference standards, detection reagents, wash buffer, chromogenic substrate, and stop solution required for the full plate run, with absorbance read on a standard microplate reader. The protocol accommodates in vitro measurement of human OXCT1 and is intended for research use only. Reconstituted standards and detection antibody should be kept frozen in single-use aliquots to limit freeze-thaw cycles, while the unopened microplate is stored refrigerated until first use. Researchers studying mitochondrial energy metabolism, ketolytic capacity in cardiomyocytes and neurons, and genotype-phenotype correlations in OXCT1 mutations will find this kit useful for protein-level quantification across sample cohorts.