Human OSF (Osteoclast Stimulating Factor 1) ELISA Kit (HUES02773)

Assay Performance & Workflow

The Assay Genie sandwich ELISA typically completes in approximately 3–4 hours at room temperature, with a standard curve range commonly spanning 0.3–20 ng/mL and a minimum detectable concentration in the low pg/mL range, enabling quantification of OSF1 across physiological and pathological samples. Each microplate kit includes pre-coated strips, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, and wash buffer concentrate sufficient for the full 96-well run. Intra-assay and inter-assay coefficients of variation are generally held below 10% and 15%, respectively, supporting reproducible comparison across patient cohorts and experimental time points.

Research Context

OSF1 expression has been documented in rheumatoid synovial tissue, periodontally affected gingiva, and osteolytic tumor microenvironments, where it contributes to osteoclast recruitment and activation. Investigators studying post-menopausal osteoporosis, multiple myeloma-associated bone destruction, or orthodontic tooth movement can use this kit to monitor OSF1 concentrations in paired serum and synovial fluid samples. For consistent results, aliquot thawed samples, avoid repeated freeze-thaw cycles, and run all standards and unknowns in duplicate on the same plate.